lectin staining to identify Haemonchus eggs

TO: WormMail  (recip undisclosed).    WormMail 2010.02.11

There have been various articles of late regarding use of peanut lectin to differentiate Haemonchus contortusfrom other strongyle eggs in faecal samples from ruminants, for example sheep.

Examples of some of these articles are pasted below.

The Oregon test is based on a publication by Dr Dieter Palmer, Senior Veterinary Immunologist and Parasitologist with the Western Australian Department of Agriculture and Fish Food?, located at the Animal Health Laboratories at South Perth (Palmer and McCombe, 1996).

Dr Palmer been in correspondence with the researchers from Oregon over the last couple of years.

Palmer and colleagues have been offering the test for many years at their South Perth laboratory. I understand that, with the aid of funding from the Australian Sheep Cooperative research Centre, they are currently simplifying the techniques and extending it to other laboratories in Australia.

Reference:

Palmer DG and McCombe IL (1996). Lectin staining of Trichostronglyid Nematode Eggs of Sheep: Rapid Identification of Haemonchus contortus Eggs with Peanut Agglutinin. International Journal of Parasitology, 42:6; 447-450.

Regards

SL      2010.02.11

Veterinarian/State worm control coordinator

I & I NSW


New lectin staining test quickly detects Haemonchus contortus

19 Jan 2010
Researchers at Oregon State University and the University of Georgia have developed an improved, more efficient method to test for Haemonchus contortus, or “barber pole” worms, a species that is very pathogenic to sheep, goats and llamas.

The new lectin staining test is based on a peanut agglutinin that binds to eggs of the parasite and can be easily visualized with a microscope using ultraviolet light. It’s an improved version of previous technology developed by scientists in Australia that was slower, less effective, more expensive and required more advanced training to perform, researchers say.

A faster, easier and less expensive way to test for the presence and quantity of Haemonchus contortus will help sheep ranchers deal with this problem more quickly and effectively, optimize their management practices, and sometimes avoid costly therapies. As with any animal health concerns, results should be reviewed with a veterinarian so that proper treatment programs can be put in place, researchers said.

The test requires only a small amount of feces, and results are available in as little as two days. Anyone interested in obtaining the test can get information on sampling, test results and fees from the Veterinary Diagnostic Laboratory at OSU (Tel:+1 541/752-5501), or Bob Storey (Dept. of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, Ga., 30602 or 706/542-0195). FAMACHA© information can be obtained through Bob Storey or by sending an email to famacha@uga.edu.

[/source]

http://www.vetsweb.com/news/new-lectin-staining-test-quickly-detects-haemonchus-contortus-825.html

26 January 2010
New test may help address costly parasite in sheep industry

Researchers at Oregon State University and the University of Georgia have
developed an improved, more efficient method to test for the most serious
of the parasitic worms in sheep, a problem that causes hundreds of millions
of dollars in losses every year to the global sheep and wool industry. This
technology is now available, and will allow a faster, easier and less
expensive way to test for the presence and quantity of Haemonchus
contortus, or “barber pole” worms, a species that is very pathogenic to
sheep, goats and llamas. This will help sheep ranchers deal with this
problem more quickly and effectively, optimize their management practices,
and sometimes avoid costly therapies. Findings about the new test were just
published in Veterinary Parasitology, a professional journal. “This
particular parasite is much more pathogenic in sheep than other worms, and
previous methods to detect it were very labor intensive and often not
commercially practical,” said Michael Kent, an OSU professor of
microbiology. “Now ranchers and veterinarians can test for this problem and
target their management or treatment strategies much more effectively.”

This parasite causes significant production losses, and in some cases it’s
the limiting factor to sheep production on pasture lands. The nematodes can
cause internal bleeding, which in turn can lead to anemia, poor food
conversion and growth, low protein levels, reduced lamb production and wool
yield, and in some cases death. Known as the barber pole or wire worm,
Haemonchus contortus is a blood-sucking parasite that pierces the lining of
the sheep’s stomach. It’s a prolific egg producer, releasing up to 10,000
eggs per day, and often causes problems in warmer climates or during the
summer. Once an infection is demonstrated, expensive treatments or complex
management strategies are often needed to address it. The new lectin
staining test is based on a peanut agglutinin that binds to eggs of the
parasite and can be easily visualized with a microscope using ultraviolet
light. It’s an improved version of previous technology developed by
scientists in Australia that was slower, less effective, more expensive and
required more advanced training to perform, researchers say.

The relatively inexpensive test was developed by microbiologists and
veterinary doctors at OSU and UGA, and is now available through those
institutions. Its use should continue to expand and become more readily
available around the world, Kent said. The test may also be of special
value to ranchers interested in organic production of sheep, goats and
llamas, who try to avoid use of chemical treatments in maintaining the
health of their animals. “One of the current testing tools commonly used by
sheep and goat farmers in dealing with H. contortus is the FAMACHA© method,
in which the farmer compares the animal’s lower eyelid color to swatches on
a card to determine the animal’s anemia status,” said Bob Storey, a UGA
researcher who co-developed the lectin staining test. “This method only
works in situations where H. contortus is the primary parasite in a given
herd’s worm population. The new lectin staining test allows for a faster
and less expensive method of determining the predominance of H. contortus
in a herd worm population, thereby making it easier for producers to
determine if FAMACHA© can be a useful tool for them. Additionally, for the
veterinarian dealing with an anemic animal and a heavy parasite burden, the
lectin staining test provides quick feedback as to whether the anemia is
parasite-based or may be due to another cause.” The test requires only a
small amount of feces, and results are available in as little as two days.
Anyone interested in obtaining the test can get information on sampling,
test results and fees from the Veterinary Diagnostic Laboratory at OSU (
http://oregonstate.edu/vetmed/diagnostic or 541/752-5501), or Bob Storey
(Dept. of Infectious Diseases, College of Veterinary Medicine, University
of Georgia, Athens, Ga., 30602 or 706/542-0195). As with any animal health
concerns, results should be reviewed with a veterinarian so that proper
treatment programs can be put in place, researchers said.

Science Daily
January 26, 2010

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